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- Expression and Detection of Different Fragments of HIV-1 Envelope Genes in a Bac-to-Bac Baculovirus Insect cell Expression System 不同区段HIV-1 env基因在Bac-to-Bac昆虫细胞杆状病毒表达系统中的表达及检测
- insect cell expression system 昆虫细胞表达系统
- The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Abstract: The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要: 昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Objective Bac-to-Bac baculovirus expression system was used to obtain biologically active recombinant human basic fibroblast growth factor(hbFGF) in insect cells. 目的利用Bac-to-Bac杆状病毒表达系统,将人碱性成纤维细胞生长因子(human basic fibroblast growth factor,hbFGF)基因进行表达,并检测重组蛋白的特异性及生物学活性。
- baculovirus/insect cell expression system 杆状病毒表达系统
- mammalian cell expression system 哺乳动物细胞表达系统
- Keywords Human papillomavirus 58;major capsid protein, minor capsid protein;virus like particles;Bacmid virus vector-insect cell expression system; 人乳头瘤病毒58型;主要衣壳蛋白;次要衣壳蛋白;病毒样颗粒;杆状病毒昆虫细胞表达系统;
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- In this paper, a MTT-based semiautomated colormetric assay was used to study the sensitivity difference among eight cultured insect cell lines to Methomyl pesticide. 采用MTT法，研究了灭多威农药对八种昆虫离体细胞系敏感性的差异。
- These indicated that pieces of E. coli and pieces of insect cell that expressed 16L1 proteins could replace purified HPV16L1 proteins as antigen in ELISA test. 表明用表达16L1的大肠埃希菌破碎物和表达16L1的昆虫细胞破碎物可以代替纯化的HPV16L1蛋白作为抗原进行ELISA试验。
- Summers M D,Smith G E.A manual of methods for baculovirus vectors and insect cell culture procedures[J].Texas Agricultural Experiment Station Bulletin,1987,1555-1556. 洪华珠彭建新.;一株高水平表达重组蛋白昆虫细胞系的建立[J]
- CBF1 expressing stable Kusa-A1/CBF1 cell line was generated by the Flp-In mammalian expression system. 方法采用基因转染法建立CBF1稳定过表达细胞系Kusa-A1/CBF1,检测其成骨活性。
- Then rAcV-Bac-bFGF was infected Sf21 insect cells again to express the hbFGF gene, its expression product was measured by SDS-PAGE and Western blotting. 进一步将重组病毒感染Sf21细胞,在Sf21细胞中进行表达,对表达产物进行SDS-PAGE电泳、Western bloting分析;
- Part of the immune system,B cells express antibodies on their surfaces that bind to particular infectious particles. B细胞作为免疫系统的一部分，其表面具备了与某种传染粒子结合的抗体表型。
- Most of CgA positive cells expressed Survivin. CgA阳性细胞多表达Survivin。
- In the adherent culture, the virus did not result in cell lysis after the insect cells were infected. 而在本论文中，我们尝试将此一非溶裂昆虫细胞/杆状病毒表现系统转移到旋转瓶的悬浮培养。
- AIM:To establish recombinant HEK29316 cell strain expressing chimera receptor EpoR/LR-F3/HAb18GEF via site-specific integration expression system. 目的:构建EpoR/LR-F3/HAb18GEF嵌合受体,并在HEK293-16工程细胞株中定点整合表达。
- Part of the immune system, B cells express antibodies on their surfaces that bind to particular infectious particles. B细胞作为免疫系统的一部分，其表面具备了与某种传染粒子结合的抗体表型。
