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- hyperfrequent endonucleases 超多位点内切酶
- hyperfrequent endonuclease 超多位点内切酶
- Any of several enzymes, such as endonucleases and exonucleases, that hydrolize nucleic acids. 核酸酶水解核酸的酶，如核酸内切酶和核酸外切酶
- The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases. 3个叶绿体和3个线粒体通用引物扩增出的片段，用15种限制性内切酶对其进行酶切。
- SeMNPV; Chinese strain; isolation and identification; bioassay; restrict endonucleases analysis; polyhedrin; chitinase; alignment of homology. 01甜菜夜蛾核多角体病毒;中国分离株;分离鉴定;生物测定;限制性内切酶分析;多角体;几丁质酶;同源性比较。
- Methods: Cutting the pGEM-7Zf -DAF plasmid with restriction endonucleases,and obtaining the recombinant human DAF gene which containing ICAM-2 promotor fragment. 方法:双酶切本室已构建质粒pGEM -7Zf-DAF ;得到含人ICAM -2启动子及DAFcDNA序列的插入片段 (3.;7Kb) ;
- Some restriction endonucleases , cleave DNA at random, but a particular group of enzymes, known as class II restriction endonuclearses, cleave DNA at special sits. 一些限制性内切酶的切割DNA的作用是随机的，但是相当一部分的内切酶，如限制性内切酶II，就是在特定的位置切割DNA。
- Its genome was composed of over 14 DNA molecules,which were different in molecular size and abundance. Molecular size of the genome was approximate 108 kbp calculated by patterns from six restrict endonucleases digestion. 琼脂糖凝胶电泳显示MmPDV基因组至少由大小不同、丰度不等的 14个DNA分子组成 ,用 6种内切酶 (EcoRI,HindIII,BssHII,PstI,BamHI,BglI)酶切MmPDV基因组后 ,估算出MmPDV基因组大小约为 10 8kb。
- The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases. 对其它腺病毒的研究表明,对六邻体蛋白基因的克隆、表达、纯化将有利于腺病毒的免疫学研究,纯化了的蛋白进一步进行活性鉴定,可用作抗原,用于动物免疫,并检测其所产生的抗体。
- The normal size of the fragments from the plasmids cut by endonucleases: The results of restriction endonuclease analysis and agar electrophoresis indicated that size of plasmids pMSCV, CED-4pMSCV, pGAG-POL and pVSV-G was normal. 质粒DNA酶切片段大小正常：酶切质粒、电泳结果表明，pVSV-G, pGAG-POL, pMSCV,CED-4pMSCV 质粒DNA大小正常；
- restriction endonucleases from Haemophilus influenzae 流感嗜血杆菌释放的限制核酸内切酶
- any of several enzymes,such as endonucleases and exonucleases,that hydrolize nucleic acids 核酸酶，水解核酸的酶，如核酸内切酶和核酸外切酶
- The Study of the Plasmid and Restriction Endonucleases Map of Y. Pestis in the Northwest Pastures in Jilin Province 吉林西北部草原鼠疫菌质粒及酶切图谱的研究
- Keywords denaturing plasmids;restriction endonucleases;gelose gel electrophoresis; 碱变性;质粒;限制性内切酶;琼脂糖凝胶电泳;
- Transcription of A Mitochondrial GIY-YIG Family Homing Endonucleases Gene Enhanced in Dunaliella salina Under Salt Stress 盐藻线粒体GIY-YIG族归巢内切酶基因受到盐胁迫时增强转录
- Roberts R J.Belfort M.Bestor T A nomenclature for restriction enzymes,DNA methyltransferases,homing endonucleases and their genes 2003(7 郭建顺.;沈晓峰
- Endonucleases 内切核酸酶类
- restriction endonucleases 限制性内切酶
- pneumoniae producting ESBLs. The genes encoding ESBLs were amplified by polymerase chain reaction (PCR). The target amplification fragment was digested with two restriction endonucleases and then cloned into pUC19 vector. 用双纸片法初筛检测产ESBLs菌株 ,聚合酶链反应扩增ESBLs编码基因片段 ,并将其克隆至pUC19载体中 ,双脱氧链终止法测定核苷酸序列以确定亚型。
