您要查找的是不是:
- Conclusion The hybridoma cell line may be applicable to the diagnosis of HBsAg. 结论筛选出了一株适合临床应用的单抗杂交瘤细胞株。
- The hybridoma cell strain E3E6 secreting monoclonal antibodies was screened and clo ned by indirect ELISA. 通过用间接ELISA试验筛选、克隆出分泌抗RHDV单克隆抗体的杂交瘤细胞林E_3E_6。
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 结果 得到1株稳定分泌抗体、效价高的杂交瘤细胞株,命名为SU-2000。
- Yuanxing Zhang, Wangfun Fong, Pingpei Yung (1998) Optimization in hybridoma cell culture. 张立,沈红,张元兴*(2000)在动态和静态培养条件下杂交瘤细胞的生长和代谢。
- The hybridoma cell lines could produce the McAbs which was specific to BDV P24 and P40 antigens with the appropriate titers. 杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。
- The monoclone hybridoma cell line obtained was named SRBC-C, which secreted anti-human B grouping red cell antibody and was used for ABO blood typing. 该株能分泌抗B抗体,与B型红细胞发生凝集,对鉴定ABO血型有一定价值。
- The effects of glucose, L-glutamine, ammonia and lactic acid on the growth of mouse-mouse hybridoma cell line G_5F_4 were studied. 研究了葡萄糖、谷氨酰胺、氨和乳酸对鼠鼠杂交瘤细胞G_5F_4生长的影响。
- The results showed that compared with myeloma cell, marked changes were not observed in hybridoma cell in cell energy and cell appearance. 结果表明,与凯骨髓瘤细胞相比,杂交瘤细胞的细胞活力和细胞形态无明显变化;
- The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line. 所述的保藏号为CGMCCNO.;1908的杂交瘤细胞株用于分泌抗URG11单抗的杂交瘤细胞株。
- The chromosome number of post-fused hybridoma cell was near to the cumulative chromosome number of splenic cell and myeloma cell. 当脾细胞与凯骨髓瘤细胞融合形成杂交瘤细胞后,其染色体数目接近两亲本细胞染色体数目之和。
- RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. 结果: 应用脾脏埋植法和杂交瘤技术, 获得4 株针对恶性疟原虫HRP-II单一表位的杂交瘤细胞。
- In 1. 5 L Celligen bioreactor, when pluronic F-68 concentration is 0. 10%(W/V) in medium and agation speed is 70r/min the hybridoma cell can grow normally. 在1.;5升GelliGen生物反应器中;培养基添加0
- Conclusion Plasmodial aldolase has been successfully expressed and purified, and the established hybridoma cell lines can secrete McAbs specific to the aldolase of P. falciparum. 结论 本实验构建并表达了重组疟原虫糖酵解醛缩酶,并获得特异性的单克隆抗体。
- The present invention discloses a mouse anti-human CD41 monoclonal antibody hybridoma cell system, a monoclonal antibody and an immunohistochemical kit and an application thereof. 本发明公开了一种鼠抗人CD41单克隆抗体杂交瘤细胞系、单克隆抗体、及其免疫组化试剂盒和应用。
- The average numbers of chromosomes of the two hybridoma cell lines were 98 and 101 which were consistent with the total chromosome numbers of spleen cell and myeloma cell. 染色体计数分析结果显示,两株杂交瘤细胞的平均染色体数分别为98和101,在脾细胞与 SP2/0 细胞染色体数之和的范围之内;
- Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen. 目的探讨在建立了分泌抗鼠疫F1抗原的单克隆抗体杂交瘤株后,对其进行克隆化的实验方法。
- McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. 再将此分离出的杂交瘤细胞,注入其他小鼠腹腔又制得腹水。
- The McAbs anti TAP were obtained by screening with indirect ELISA and 4 times clone, and were to be preliminary characterized. Results The rate of fusion was 85.03%. Ten hybridoma cell lines were developed which could secret McAb stably. 结果细胞融合率85.;03%25;经4次克隆化后获得10株能稳定分泌TAPMcAb的杂交瘤细胞株;经免疫球蛋白亚类鉴定;除1株为IgG1外;其余9株均为IgM类;
- The cTnI was used to immunize Balb/c mice by route method. The spenocytes were fused with SP2/0 myeloma cell lines. Six hybridoma cell lines,secreting stably McAbs to cTnI antigen have been obtained and screened. 方法 :应用cTnI免疫Balb/c小鼠 ,取脾细胞与SP2 /0骨髓瘤细胞进行细胞融合筛选 ,获得分泌高效价抗cTnI抗体细胞株 6株。
- Results: Hybridoma cells growed well in 12 spots with DMEM culture.And of the 12 spots,6 spots chromosomes had good shapes and stead numbers. 结果:12孔板中加入DMEM培养液的杂交瘤细胞株生长良好,其中6孔板加秋水仙素培养1h,染色体形态最佳,数目稳定。