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- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering. 在复苏后早期阶段ES细胞培养中 ,不宜单独应用BRL条件培养基 ,须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层
- MEF, bEF, hEF feeder layer cells all can promote the efficiency of the isolation and clone human ES-like cells derived from PGCs. MEF、bEF、hEF三种饲养层细胞对人PGCs体外培养均有促进作用。
- CONCLUSION: Mouse blastula can develop into embryonic stem cells on the fibroblast cell feeder layer, and can be passaged. 结论：小鼠囊胚在胚胎成纤维细胞饲养层上可发育成胚胎干细胞，并能进行传代。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词：胚胎成纤维细胞；
- An integral boundary layer method based on the dissipation integral is used to account for viscous effects. 考虑附面层影响，通过粘/位迭代得到跨音速翼/体的非定常气动力。
- A integral boundary layer method based on the dissipation integral is used to account for viscous efforts. 考虑附面层影响，通过粘位迭代得到跨音速机翼操纵面的定常、非定常气动力。
- CONCLUSION: Endometrial fibroblast is considered to be feeder layer of PGC.After passage, PGC remains undifferentiation and pluripotent of embryonic stem cells. 结论：人子宫内膜成纤维细胞可以作为人原始生殖细胞的饲养层细胞，传代后的原始生殖细胞仍保持胚胎干细胞的未分化状态和发育全能性。
- MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer;growth of embryonic stem cells;results of alkaline phosphatase staining;cell differentiation tendency. 主要观察指标：成纤维细胞饲养层的制备情况，胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。
- The technology of pulling forming method of helical vane is introduced. Accurate vane materiol drawing is made by middle layer method. 介绍螺旋叶片拉伸成形工艺方法。以及如何采用中间层计算法准确计算螺旋叶片下料图尺寸。
- The thin layer method(TLM) is a semi-analytical and semi-numerical approach for the analysis of elastic wave propagation in stratified media. 薄层法是分析和模拟弹性波在层状介质中传播的一种半解析半数值方法。
- Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs. 在适当的条件下;可以形成饲养层细胞;为造血干/祖细胞体外扩增提供支持基质细胞.
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- When cultured on degenerated feeder layers or in suspension, EG cells formed embryoid bodies (EBs) in vitro. 当EG细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现EG细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构.
- Keywords mouse;feeder layer;method of trypsinization; 大鼠;饲养层;胰酶消化植块培养法;
- The effect of Acrylonitrile-Butadiene's contents in EPDM rubber inhibitor formulations on the inhibitor's performance of resisting nitroglycerin migration is investigated by intermediate layer method. 采用夹层法研究了丁腈橡胶含量对三元乙丙橡胶包覆剂抗硝化甘油迁移性能的影响。
- METHODS: PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture. 方法：从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
- The method seems good but it needs to be tried out. 这个方法似乎不错，但需要试验一下。
- Based on the algorithm of 2 dimensional FEMOL,with the essential thought of Finite Layer Method(FLM),a new scheme of 3 dimensional semi analytic algorithm is developed in this paper. 在平面有限元线法的基础上,结合有限层法的分层离散、层内解析的思想,在三维空间中构造出了系统的半解析算法。
- In this procedure, an evaluative stage joins the RUP kernel recycling stages and the layered method is used to make the work of needs analysis more accurate and feasible. 在此过程中，根据实际要求，为RUP规定的核心工作流中额外加入了评价活动，并采用分层处理的方法来完成需求分析阶段的工作，从而提高了开发系统过程准确性和可行性。
