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This cocultivation method may become a new one to supply seed cells for bone tissue engineering. 该方法可能成为一种新的骨组织工程种子细胞的获取方法。
ACP content within LAK cells at 60, 90, 120, min after cocultivation was significantly higher than that of the control group. LAK细胞内ACP酶在60、90、120分钟时酶含量较高;与对照组相比差异非常显著(P<0.;01)。
After coculture with fibrin. mRNA expression of tPA. uPA and PAl-1 in GEC elevated significantly. 纤维蛋白能够明显促进tPA、uPA与PAI－1的mRNA表达上调。
The bacterial cell density and the period of cocultivation of the different genotype apple had significantly effect on transfer efficiency. 苹果基因型、菌液浓度和共培养天数对不同基因型苹果的转化效率影响很大。
Its further advancement has been promoted by the coculture technique and application of new biomaterials. 各种细胞共培养技术的改进和新型载体材料的应用也有力地推动其进一步完善。
Results: The growth of neurons was good, the neuraxons extended and formed network in coculture of OECs and neurons. 结果神经元与嗅鞘细胞共培养下神经元生长良好,轴突延长并形成网络状。
Appearance rate of 16cell mouse embryo was 32.5% in EMT group versus 47.6% in control group after 48 hour coculture with peritoneal fluids(P<0.01). 体外培养 48h后 ;两组小鼠 2细胞期胚胎发育成 16细胞期胚胎的比例分别为32 .;5%25和 47
The values of alanine aminotransferase(ALT) and lactate dehydrogenase(LDH) in coculture supernatants of H. pylori and HepG2 were increased. 并且培养细胞的上清液出现谷丙转氨酶和乳酸脱氢酶的增高。
Results Mouse embryos were successfully hatched, adhered, implanted and outspreaded under the coculture system.The extraplacental cone and amniotic sac were formed as well. 结果小鼠胚胎在体外无血清培养体系中能够顺利孵化、粘附、种植及外延生长，并形成外胎盘锥和羊膜囊；
In vitro, the coculture of biomaterials and cells is used to reflect biomaterials' cell toxicity by detecting cells' morphology, viability, apoptosis and so on. 体外评价即利用生物材料与细胞共培养，通过观察细胞的形态，测定细胞的活力和凋亡等反映细胞的细胞毒性。
Primary cultures of macrophages and endometrial stromal cells respectively and coculture were conducted and the two cell types were identified by immunocytochemistry. 两种细胞单独培养与共培养，并用免疫细胞化学鉴定；
The optimized condition for transformation triploid Chinese white poplar were co-cultured for 2 days and infected 10-15minutes with Agrobacterium concentration of OD600=0.3. It is benefit to obtain kmr shoots in the coculture medium without CoCl22H2O. 同时还发现共培培养基中去除微量元素的CoCl_22H_2O，转化频率由2.;9%25升高到6
The sensitivity of transfected cells to GCV and "bystander effect (BSE)" of HRE-TK/GCV system under normoxia or hypoxia conditions were measured by MTT assay and mixed coculture experiment. 用MTT法和混合共培养实验分别检测转基因细胞在缺氧或不缺氧状态下对GCV的敏感性以及HRE-TK/GCV系统的“旁观者效应”;
After coculture for 24 h, the proliferation of HSCs was inhibited to a moderate extent by MSCs. With the coculture time lasted, the significant growth suppression of HSCs was affected by MSCs (15.7% and 30.3%) but not BRL cells in 48 h and in 72 h. HSCs与MSCs共培养24h;HSCs表现轻度增殖抑制;随着培养时间延长;HSCs增殖活性受抑制更明显;在48h和72h的抑制率分别达15.;7%25与30
In a VSMCIEPC coculture system the adhesive ability of EPCs was examined by adhesion experiment and the number of clone forming units and the morphorlogy of EPCs were observed by a microscope. 建立内皮祖细胞和平滑肌细胞的联合培养模型；细胞粘附实验测定内皮祖细胞的粘附能力；形态学观察内皮祖细胞的分化与增殖。
Keywords Stem cell;Smooth muscle cell;Differentiation;Coculture; 干细胞;平滑肌细胞;分化;共培养;
Keywords rats;germ cells;coculture techniques;spermatogenesis; 大鼠;生殖细胞;共同培养技术;精子发生;
Keywords Cell adhesion;Living bone compounds;Osteoblasts;Coculture;Scaffold; 细胞粘附;活骨组织;成骨细胞;共培养;支架;
Keywords Adhesion;Shear stress;Coculture;Vascular smooth muscle cells;PKB/Akt; 细胞粘附;切应力;联合培养;血管平滑肌细胞;蛋白激酶B;
Keywords hypoxia;coculture;pulmonary artery;smooth muscle cells;interleukin 6; 低氧;复合培养;肺动脉;平滑肌细胞;白细胞介素-6;