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antisense Gene transfer 基因转移,水平
Viruses can be used as gene transfer vehicles. 细菌可用作基因转移的媒介。
Meanwhile, the application of antisense gene technology is prospected. 并对反义基因技术的应用进行了展望。
Rat antisense AT 1B gene was succesfully transducted into the carotid artery after carotid balloon injury. Neointima/media area ratio was significantly reduced (47%, P <0.01) compared with the control group at day 21 after gene transfer. 用腺病毒载体转导大鼠反义AT1BRNA至大鼠球囊损伤的颈动脉 ;与对照组相比 ;2 1天后损伤血管新生内膜 /中层面积比降低了 4 7%25(P <0 0 0 1)。
Somatic gene transfer only targets body cells (somatic cells). 体细胞基因只能被转进体细胞。
We found that ATF-lys10 mediated uPAR antisense gene delivery could downregulate uPAR protein level as well as adhesion ability to fibronectin. 结果发现，ATF-lys10介导的uPAR反义核酸基因转移可以下调细胞uPAR蛋白水平，并降低细胞与纤连蛋白的粘附能力。
Objective To produce antisense vascular endothelial growth factor (VEGF) adenovirus expression vector for the antisense gene therapy of RA with antisense VEGF gene. 目的制备反义血管内皮生长因子(VEGF)腺病毒表达载体,为反义VEGF转基因治疗类风湿关节炎(RA)奠定基础。
As a mammalian gene transfer vector, baculovirus owns some unique advantages. 杆状病毒作为哺乳动物细胞基因转移的载体有很多独特的优点。
Horizontal gene transfer (HGT) is a cross-species form of genetic transfer. 横向基因迁移是指跨物种形式的遗传迁移。
Results of PCR amplification and restriction digestion showed that the fragment of antisense gene CYP86MF was introduced into pBI121 plasmid and the expression vector pBI35S-AMF was transferred into Agrobacterium successfully. PCR扩增和酶切鉴定结果表明;所构建的反义CYP86MF基因植物表达载体pBI35S-AMF是正确的;并已成功导入了根癌农杆菌中.
The expression of K-RAS P21 protein in AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells was obviously lower after the transduction of specific K-RAS Antisense gene,which has little impact on BxPC-3 pancreatic carcinoma cells. 转导K-RAS突变特异性反义基因后,AsPC-1和MiaPaCa-2胰腺癌细胞的K-RAS P21蛋白表达明显降低; BxPC-3胰腺癌细胞的K-RAS P21蛋白表达无明显变化。
Effect of eNOS Gene Transfer on the Capacity of Hepatic Sinusoids in cirrhotic rats. eNOS基因治疗对肝硬化大鼠肝窦容积的影响
Latter, we observed the effects of ATF gene transfer on invasive potential of cancer cells in vitro. 观察ATF基因转移对肿瘤细胞体外侵袭人工基底膜能力的影响;
Copolymer PEG-PEI(2-25-1) was proved to be a better carrier for in vitro gene transfer. 共聚物PEG-PEI(2-25-1)被证实为较有效的介导体外基因传递的复合物。
The effect of human interleukin 2 gene transfer on the adhesive ability of ovarian cancer cell line SKOV3. IL-2基因转移对卵巢癌细胞系SKOV3粘附性的影响
Compared with A549cells,the pHi of c ells co-transfected antisense genes was remarkably decreased(P<0.05),while lactate content was remarkab ly increased(P<0.001). The cell cycle was blocked at G 0 -G 1 period. 与A549细胞比较;联合转染反义基因的细胞pHi降低、乳酸显著升高(P<0.;001);细胞周期阻滞在G0-G1期。
Objective:To investigate the efficiency of cationic liposome in mediating gene transfer. 目的:研究阳离子脂质体介导体内外基因转移的效率。
Gene transfer is achieed using a ector ? a kind of ehicle that carries the gene. 基因转染是通过载体实现的，载体就是运载基因的工具。
Horizontal gene transfer plays a special role in genetic, ge-nomic, and the biological evolution. 基因水平转移在基因、基因组和生物进化中有着其独特的作用。
Gene transfer vector is the important ingredient in gene therapy,which includes virus vector and non-virus vector. 基因转移载体是基因治疗的重要组成部分,包括病毒载体和非病毒载体。