With rational design of fed-batch culture processes,hybridoma cells were grown under(nutrient-fortified,) glucose-limited and glutamine-limited conditions to typical pseudo-steady states,respectively.

• 采用流加培养方式,实现了杂交瘤细胞在营养物富裕、葡萄糖限制和谷氨酰胺限制3种条件下的拟稳态培养。