When the ratio of BSA-GoldMag complex and the polyclonal antibody was 7 :1 and reacted more than 2 hours at 4 C ,the antibodies against BSA can be removed effectively while the titration of antibodies against CBL remained at the same level.

• 在最佳纯化条件(温度4C,反应时间2h,金磁微粒表面BSA质量与抗体质量比为7:1) 下,金磁微粒-BSA可有效地去除偶联物多克隆抗体中的抗BSA抗体,而纯化前后抗CBL抗体的效价基本保持不变,分离纯化效果较好。