When embryos co-cultured with GCs in media SOFaa, the highest blastocyst rate was obtained from supplement with 10% BFF (25.1%) which was significantly different from 5% BFF group (12.9%) and 15% BFF group (3.0%).

• 在SOFaa培养液与颗粒细胞共培养条件下；添加10%25BFF的ICSI胚的囊胚发育率(25.；1%25)显著高于添加5%25(12