When antibody titer tested by ELISA against PRV gE protein reached over 1:25600, monoclonal antibody (McAbs) were prepared by fusing SP2/0 myeloma cells with spleen cells of immunized mice.

• 基因免疫接种小鼠股四头肌，接种前用盐酸普鲁卡因作预处理，用间接ELISA的方法检测小鼠血清抗体，当血清抗体效价达到1：3200以上时，进行细胞融合，融合前不作加强免疫；