We observed thatthere is direct interaction between p300 and transfactors C/EBP or c-Fos. Then, weidentified the existence of an interaction of IL-12 p40 promoter with c-Fos and C/EBP.

• 进一步通过DNAPull-down实验并结合了免疫共沉淀实验证实, p300 能乙酰化 C/EBP 且与转录因子 C/EBP、c-Fos直接作用,同 IL-12 p40 启动子形成复合物来参与调控该启动子的转录活性。