We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1).

• 本实验利用荧光漂白后恢复(FRAP,fluorescencerecoveryafterphotobleaching)法观测人肺腺癌肿瘤细胞(ASTC-a-1)中表达的27kDaEGFP在核质间的被动扩散,并以激光共焦显微镜进行实时成像。