Vein blood samples 4-6ml were collected and Genomic DNA were extracted. Exon 9 of CFH gene was PCR amplified .
英
美
释义
抽取外周抗凝血4-6ml,用酚-氯仿抽提法提取全基因组DNA,聚合酶链反应(Polymerase chain reaction ,PCR)扩增外显子9片断并进行直接测序。
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