Validation analysis showed that ligation direction of the scFv gene of Pichia pastoris and the Fc fragment DNA was correct and was in agreement with the correct reading frame of scFv-Fc.

• 序列分析表明,scFv基因与Fc片段基因拼接正确,阅读框正确无误,表明成功构建了抗脂肪细胞40000特异膜蛋白scFv-Fc融合抗体毕赤酵母表达载体。