VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. ATi protein expression was determined by Western Blotting.

• 裂解细胞，考马斯亮蓝G一250染色法测定蛋白浓度，使用节范stem Blotting法检测ATI蛋白表达量。