Using the saliva supernatant, they isolated RNA, performed two-round linear amplification with T7 RNA polymerase, and applied human genome U133A microarrays for profiling human salivary transcriptome.

• 使用唾液离心后的上清液，他们分离出RNA，以T7 RNA聚合脢进行两个回合的线性放大作用，以及应用人类基因体U133A微阵列来分析人类唾液转录体。