Using packed bed reactor filled with immobilized cells to produce D-mandelic acid methyl ester, 25 days later the conversion efficiency can be still sustained in 80% and the ee value is more than 95% when the charge was 0 2 mmol/(d·g) wet cell.

• 采用固定化细胞填充床反应器转化苯乙酮酸甲酯 ；每克酵母湿菌体进料负荷为 0 2mmol d ；成功运行 2 5d ；转化率保持在 80 %25以上 ；D 扁桃酸甲酯ee值达 95 %25以上 .