Using SOFaa as the culture medium and adding FBS, OGS, BFF to the co-culture system respectively, we found that the blastocysts rates were 27.72%,48.90%, and 46.83% . It implied that OGS or BFF is more profitable for the co-culture system than FBS.

• 以SOFaa为基础培养液；分别添加FBS、OGS和BFF；结果囊胚率分别为27.；72%25；48