Using PCR, the Sox genes of Rana nigromaculata and Rana plancyi were amplified with a pair of primers, which can also amplify human SRY.

• 采用PCR技术，以参考人SRY基因HMC-box的保守区序列而设计一对特异引物，扩增了黑斑蛙和金线蛙的Sox基因。