Use RPMI basic medium supplemented with heat-inactivated 10% FBS, culture under 37?, saturated humidity and 5%CC>2.

• 将细胞悬液置于56OC灭活的10%25胎牛血清＋RPMI－1640液的培养体系中，37℃、5%25二氧化碳、饱和湿度条件下培养。