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- 1. To culture human conjunctiva fibroblast cells by trypsin digestion method and describe their growth features in vitro. 方法:1、用胰蛋白酶消化法取材人结膜成纤维细胞,并描述人结膜成纤维细胞的生物学特性。
- The best digestion time determined by trypsin digestion method for collecting neural stem cells derived from spinal cord 胰酶消化法收集脊髓神经干细胞最佳消化时间的选择
- Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. 方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。
- Methods: Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. 方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。
- The whole spinal cords of neonatal rats were dissociated and single cells were separated by trypsin digestion. 本研究探索利用新生大鼠脊髓培养和纯化少突胶质细胞的方法。
- Methods Cardiomyocytes and cardiac fibroblasts were isolated by trypsin digestion method. DNA and protein synthesis were measured by 3 H-deoxythy-midine( 3 H-TdR) and 3 H-Leucine( 3 H-Leu) incorporation. Protein content was measured by Bradford method. 方法采用体外乳鼠心肌细胞和心脏成纤维细胞培养,一定浓度的ET-1、ANP单独或联合作用细胞24h,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法测定细胞DNA、蛋白合成,Brad-ford法测细胞总蛋白含量,RT-PCR法测心肌细胞心钠素ANPmRNA的表达,评价ET-1、ANP对心肌细胞和成纤维细胞的作用。
- N-and C-half molecules containing a single iron-binding site were simultaneously obtained from trypsin digest of iron-saturated pig transferrin. 摘要用胰蛋白酶水解铁饱和的猪血清转铁蛋白,可同时获得含单一铁结合部位的N端和C端半分子。
- Trypsin digestion was used in the subculture of mRTECs.The types of cells were identified by cellular immunocytochemistry. 胰蛋白酶消化传代,以免疫细胞化学方法鉴定细胞种类。
- The purpose of the study is to compare the traditional acid method. incineration method and microwave digestion method. 本文比较了三种样品消化方式,即传统酸消化法、灰化法及微波消化法。
- Clear and abundant G-banding at late prophase,prometaphase and early metaphase along the length of the tea chromosome has been induced by trypsin digestion. 摘要 本文应用胰酶法在茶树的晚前期、前中期和早中期的每一染色体的全长上诱导出了清晰而丰富的G带带纹。
- The direct comression and artificial digestion method were used to examine the encysted metacercaria of C. 用直接压片法和消化法检查淡水鱼虾体内的华支睾吸虫囊蚴。
- Conclusion Collagenase and trypsin digestion assisted by immuno-magentical column isolation is effective for isolation of type-A and type-B synoviocytes. 结论胶原酶和胰蛋白酶联合消化,免疫磁柱法是一种确切有效的分离人类关节滑膜A、B型细胞的方法。
- A microwave digestion method was developed for the determination of COD Cr in refinery sewage with Ag 2SO 4-CuSO 4 catalyst. 以 Ag2 SO4-Cu SO4为催化剂 ,探讨了微波消解测定炼油污水中化学需氧量 (Chemical Oxygen Demand,CODCr)的方法。
- The direct comression and artificial digestion method were used to examine the encysted metacercaria of C.Sinensis in freshwater fishes. 用直接压片法和消化法检查淡水鱼虾体内的华支睾吸虫囊蚴。
- Digest the soil sample by waterbath digestion method with colorimeter.Detect mercury in soil by atomic fluorescence spectrometry. 采用比色管水浴消解法消解土壤样品,以原子荧光光度法测定土壤中的汞。
- The Prev-DAF Testing Kit was used to detect mtDNA A1555G mutation.The GJB2 235delC mutation was identified by restricted enzyme Apa I digestion method. 为减少社会和家庭的负担,进行聋病的分子流行病学调查显得非常重要,根据调查结果可以正确指导产前检查,从而减少聋儿的出生率。
- The embryonic lung cells were isolated by trypsin digestion of a human embryonic lung obtained from a therapeutic abortion case and was designated as cell line Elu 8801. Anopheles stephensi mosquitoes were infected by biting P. b. 用胰酶消化法自人工流产胎儿分离出胚肺细胞后,建立Elu 8801细胞株。
- The result of determining the content of heavy metals of Ca,Cr,Cu Ni and Zn is identical with that of the normal digestion method(HNO 3-HClO 4. 重金属元素镉、铬、铜、镍、锌含量测定的结果,与用常规消化方法(硝酸-高氯酸)相吻合。
- In the present study, the hepatocytes were obtained through shearing 9-day-old Sanhuang chicken embryo liver tissue, separating by trypsin digestion and culturing in revised M199 culture. 摘要剪切9日龄三黄肉鸡鸡胚肝组织,胰酶消化分离肝细胞,用改良的M199培养液进行培养。
- Conclusion Detection of SMN1 gene deletions with the PCR-enzyme digestion method can be served to diagnose SMA patients accurately and quickly. 结论应用PCR-酶切法检测SMN1基因缺失从而进行SMA患者的基因诊断,具有准确、简便和快速的优点。