Trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotinin from the extract of cattle lungs by affinity chromatography.

• 采用化学改性的壳聚糖为载体,共价法偶联胰蛋白酶,制成抑酶肽亲和吸附剂,将其直接亲和层析牛肺提取液,分离、纯化高比活力的抑酶肽。