Total serum bile acids were extracted and purified bySEP-PAK C18 Cartridge and were grouped with anion exchange resin PHP LH-20, conjugated bile acids were converted to free form by alkaline hydrolysisunder high pressure and temperature.

• 血清胆汁酸经SEP-PAK C_(18)小柱固相提取、净化,用PHP LH-20柱进行分组,结合型的胆汁酸在高温高压下水解为游离型。