Total RNA of avian viral arthritis virus C-98 strain(AVAV C-98) isolated in Nei Monggol,China was extracted. According to the published S1 gene sequence of ARV-S1133 strain in GenBank,two pairs of primers were designed.

• 提取鸡病毒性关节炎病毒(AVAV)内蒙古分离株(C-98株)总RNA,参考GenBank中禽呼肠孤病毒(ARV)S1133株S1基因序列设计了2对引物,应用RT-PCR技术扩增了病毒S1基因,将S1基因cDNA克隆到pGEM-T Easy载体后测序。