To understand whether K562 cell was able to produce the acquired resistance to verapamil(VER),a model of K562 cell subline resistance to VER(K562/VER) was established through gradually increasing VER concentration in the media.

• 为探讨人白血病细胞对耐药逆转维拉帕米的耐药机制，我们通过不断提高培养液中维拉帕米浓度建立维拉帕米耐药性K562亚系（K562/VER），采用MTT法测定维拉帕米、潘生丁和环孢素A对K562、K562/VER细胞的毒性或耐药性；