To test whether DNA methylation plays a role in somaclonal variation, the calli were treated with 5-azacytidine that reduces cytosine methylation by blocking activity of DNA methyltransferase.

• 为了检测DNA甲基化在体细胞克隆多样性中的作用，愈伤组织用5-氮胞苷进行处理，5-氮胞苷通过抑制DNA甲基转移酶的活性降低胞嘧啶的甲基化水平。