To strip away periostea from calvaria by enzymes digesting separation and then culture explant in DMEM supplemented with 10% fetal calf serum.The new method is named floating explant culture.

• 在常规培养方法的基础上进行改良，建立了组织块漂浮培养法，从新生兔颅骨分离、培养成骨细胞。