To examine the feasibility of she-specific integration strategy based on Flp recombinase mediated cassette exchange at mouse HPRT locus, exchange plasmid pF-HPRT-F3, Flp recombinase expression plasmid pCMV-Flp and pEF-Flp were constructed.

• 利用获得的重组ES细胞、构建成的交换质粒pF-HPRT-F3及Flp表达载体pCMV-Flp和pEF-Flp，本研究进行了Flp重组酶介导的盒式交换试验。