To construct the recombinant SFV containing the PrM E gene, the SFV expression vector DNA, which contained the PrM E gene of Chinese dengue 2 virus, and the helper vector DNA were linearized and transcribed in vitro , respectively.

• 为构建含PrM E基因的重组SFV病毒 ；将含我国登革 2型病毒PrM E基因的SFV表达载体和辅助载体DNA线性化后 ；进行体外转录 .