To construct recombinant adenovirus containing the capsid(P1) and 3CD protease coding regions of O-type foot-and-mouth disease virus(FMDV),the genes of P1 and 3CD was obtained by reverse transcriptase-polymerase chain reaction(RT-PCR).

• 目的构建包含有O型口蹄疫病毒(FMDV)强毒结构蛋白(P1)基因及弱毒非结构蛋白(3CD)基因的重组腺病毒,为进一步研制FMD活载体疫苗奠定基础。 方法通过RT-PCR方法扩增得到含有FMDV P1、3CD编码区的目的基因。