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Establishment of tissue microarray technique by manual method 组织芯片的研制及效果
Tissue microarray techniques 组织芯片
Protein expression patterns of apoptosis and cell cycle-related genes in classical Hodgkin's lymphoma with tissue microarray technique 应用组织芯片技术研究霍奇金淋巴瘤细胞凋亡和细胞周期相关基因蛋白的表达
Objective To clarify basic application scopes of tissue microarray. 目的探索组织芯片的基本应用范围。
Immunophenotype,tissue microarray and molecular studies underline the extreme heterogeneity of DLBCL and suggest a subclassification of the tumor. 近年来,对DLBCL进行了大量免疫组化和分子生物学研究,并试图对其进一步分型。
In 12 eases of NK/T-cell lymphoma (two in the tissue microarray, ten routine sections), both perform and granzyme B were strongly positive. 芯片中的2例和常规10例鼻NK/T细胞淋巴瘤中均见穿孔素和颗粒酶B过度表达。
Biomicroarray is mainly composed of gene microarray, protein microarray and tissue microarray, which may function in identifying tumor-specific markers for gastric cancer. 基因芯片、蛋白芯片与组织芯片构成了生物芯片的主体，该技术有望在胃癌特异性标志物发现上发挥作用。
Objective To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray, and provide evidences for clinical treatment and prognosis. 摘要目的运用组织芯片及常规技术检测活化的细胞毒性细胞在各型淋巴瘤中的表达分布情况，为临床治疗和判断预后提供依据。
In addition, we compared the effects of BFP extracts and estrogen by observing the 1176 mouse cDNAs using microarray technique. 实验还应用点阵列技术观察BFP有效成份以及雌激素预处理的PD模型小鼠中脑与下丘脑的1176种基因的表达特性。
In this study, we first applied cDNA microarray technique to analyze the differentially expressed genes between 2 pairs of AAA and normal aortic tissues. 本研究首先应用基因芯片技术对2对AAA和正常主动脉组织进行了基因差异表达分析。
A study on manufacture of tissue microarray 组织芯片研制的几点体会
Clinical application of DNA microarray technique to recognition of hepatitis B and C [J].J Southeast Univ (Medical Science Edition), 2002, 21(1): 75-80. DNA芯片技术诊断乙、丙型病毒性肝炎的研究[J].;东南大学学报(医学版);2002;21(1):75-80
METHODS: Beecher tissue arrayer was used to create tissue microarray,derived from 30 cases of gastric adenocarcinoma, and 30 corresponding normal gastric tissues were used to be the controll. 方法:选用胃腺癌组织30例制备组织芯片,30例正常胃组织作为对照,用免疫组化的方法观察硒蛋白P在胃腺癌组织中的表达。
Methods:Microarray technique was used for examination of the gene expression profiles in 4 cases of cold syndrome patient and 6 normal persons in a same family. 方法:应用基因芯片技术对同一家系中的4例虚寒证患者与6名正常人进行基因表达谱检测。
METHODS:Beecher tissue arrayer was used to create a 196 dot tissue microarray, derived from 99 cases of common carcinomas, and their para cancerous tissues and corresponding normal tissues which were formalin fixed and paraffin embedded. 方法 :在病理档案材料中选取 10种常见恶性肿瘤及其癌旁或正常组织的石蜡包埋标本 ,HE染色切片定位穿刺部位 ,每一蜡块穿取直径 0 6mm大小组织柱 ,排布成 196点列阵的芯片 ;
Keywords Tissue microarray;Pathology;Teaching; 组织芯片;病理学;教学;
Detection for hantavirus in chigger mite with microarray techniques 恙螨体内汉坦病毒核酸的基因芯片检测
Objective To screen acute rejection related genes of allogeneic cardiac transplantation by cDNA microarray technique and study the mechanism of acute rejection of allogen eic cardiac transplantation in rats. 目的运用基因芯片技术分析同种异基因大鼠心脏移植后急性排斥反应的基因表达谱。
Objective: To investigate the regulation of MMP-9/TIMP-1 expressions in glyceryl trinitrate (GTN), that acts as the donor of nitric oxide, treated Hela cells with microarray technique, RT-PCR and ELISA assay. 目的利用基因芯片、RT-PCR技术和ELISA方法观察硝酸甘油(GTN)作为一氧化氮供体对人宫颈癌细胞(Hela)MMP-9/TIUP-1基因及蛋白表达的调控。
It is generally accepted that cDNA microarrays, tissue microarray (TMA) and RNA interference (RNAi) are useful tools for identification and validation of biomarkers in disease research. 人们普遍认同，cDNA 芯片、组织芯片及RNA干扰是研究中鉴别和验证疾病相关生物标记物的非常有用的工具。