Three pairs of specific oligonucleotide primers for amplifying the NP, P and M gene of NDV were designed according to the published relevant sequences of NDV, and the NP, P and M gene of a virulent NDV strain (Changchun) were amplified by RT-PCR.

• 计算机辅助设计了3对特异性引物.；应用RT-PCR一次性扩增出NDV长春株NP、P、M基因的氨基酸编码区(ORF)。