Three different extraction methods (cold methanol, hot ethanol and alkali extraction) were analyzcd and compared using the direct infusion electrospray ionization-mass spectrometry (ESI-MS) as the evaluation standard.

• 本研究以特定厌氧条件下培养的大肠杆菌为研究对象，以电喷雾质谱直接进样测定为评价标准，比较并分析了冰甲醇、热乙醇和碱抽提3种抽提方法。