This study is to delineate CpG motifs and CpG ODN for activating human immune cells. Methods A panel of CpG ODN containing two copies of CpG motifs was screened for their capacity to stimulate human B lymphocytes to secret IgM.

• 方法 设计合成含有 5′ CG 3′二核苷酸、长度为 1 2个碱基的硫代修饰CpG ODN ,并利用体外培养的人外周血单个核细胞 (PBMC) ,以培养上清中的IgM水平为指标 ,筛选能够活化人免疫细胞的CpG基序。