This inoculation technique was simple, rapid and relible and could be used as basal identification method of resistance of soybean to phytophthora root rot disease.

• 本方法所获鉴定结果稳定可靠，利用注射器接种可以极大地提高工作效率，建议作为我国鉴定大豆种质资源对大豆疫霉根腐病抗病性的基本方法。