There are a lot of shortcomings of traditional tissue culture conditions, which have been improved through some ways such as culture medium, container, environmental conditions (illumination, gas).

• 摘要植物组织培养技术具有巨大的应用价值，传统组织培养存在许多缺点，许多学者从培养基、培养容器、环境条件（光照、气体等）进行了研究和改善，取得了一定的成效。