Then, the optimization of fermentation technique was carried out both in Erlenmeyer flasks and 15 L agitating fermentor, and the amplifying of fermentation technique was implemented in 50 L agitating fermentor.

• 利用15 L发酵罐进行了发酵动力学研究；确定了发酵的最佳终止时间; 利用50 L发酵罐中进行了中试放大和发酵条件正交优化试验；结果表明较合适的发酵条件为搅拌速度300 r min~-1、通气量1:1.；00 vvm、发酵温度27。