Then transfered the callus into stress cultural base (MS + 2, 4 - D + NaCL1% + NaHCO3 0.5%)for 20 days treatment. Then transfered to differentiation base, 119 rice mate-rial(simply called D-rice)were got with adove mentioned method.

• 作法是将去壳的水稻种子经常规消毒接种在脱分化培养基诱导形成愈伤组织；继代一次；转移到加压培养基(MS+2.；4-D2mg/L+NaCl1%25+NaHCO_30