Then the biological engineering strain WYBS2001T with human egf was obtained by transforming pUSE which was constructed by cloning egf into the secreting plasmid pUSl86, into mutant strain WYBS2001.

• 然后将其克隆至枯草杆菌分泌型质粒载体pUSl86上构建成重组质粒pUSE，并转化枯草杆菌突变菌株WYBS2001，获得转入egf基因的枯草杆菌生态工程菌WYBS2001T。