Then the HA gene was sub-cloned into the vector pVAX1 at the downstream of CMV directedly. The recombinant, named pV-HA, was used as TIV DNA vaccine. F81 cells transfected with pV-HA did encode TIV HA protein specifically reacting to anti-TIV sera.

• 克隆了TIV HA基因并将其定向插入到真核表达载体pVAX中,得到基因疫苗表达载体pV-HA,将pV-HA 转染F81 细胞后,间接ELISA 试验结果表明,其能在F81 细胞中表达具有生物活性的HA蛋白。