Then a 1240bp fragment encoding gD gene was amplified by PCR technique from the genome of PRV Ea strain,which was separated from HuBei province.

• 本文依据国外有关PRV囊膜蛋白gD的研究进展，成功地克隆了我国PRV地方毒株鄂A株的囊膜蛋白gD基因，并首次运用杆状病毒载体系统进行了融合及非融合表达。