Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a general primer containing oligo-dT).

• N－端 9个氨基酸的序列，并据此设计5，端简井引物，用此引物和 3’－端通用引物，通过RTFCR技术克隆了 Luffin S。 的部分cDNA序列。