The typical problems encountered in enzyme encapsulation through sol\|gel proces s,such as too long gelation time,small gel pore size,low gel mechanical strength and so on, are briefly outlined.

• 综述了溶胶凝胶固定化酶由于凝胶化时间过长,凝胶孔径偏小以及凝胶机械强度低等问题的存在引起酶构象改变、屏蔽,底物与产物的扩散速率慢以及酶与底物的不易接触。