The two recombinant plasmids were transfected into the HepG2 cells , then the inhibition effect on HBV replication by the genome of the HBV defective virus was studyed by ELISA dot hybridization and semiquantitative PCR.

• 构建含乙肝缺陷病毒基因组的重组逆转录病毒载体和含乙肝病毒基因组的重组逆转录病毒载体，共转染HepG2细胞，ELISA、PCR方法和斑点杂交初步研究乙肝缺陷病毒基因组对HBV复制的影响。