The tissue is homogenized with chloroform/methanol (2/1) to a final volume 20 times the volume of the tissue sample (1 g in 20 ml of solvent mixture).

• 组织样本用氯仿／甲醇（2／1）试剂均质，试剂用量是样本的20倍（1克样本用20毫升的试剂）。