The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in cis and trans direction under T4 promoter, separately.

• 切下目的片段与酶切的穿梭质粒pJFF224-XN连接,经PCR方法及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下;