The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in ?cis? and ?trans? direction under T4 promoter, separetely.

• 切下目的片段与酶切穿梭质粒pJFF224-XN连接,经PCR及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下。