The smaller truncated P32 fragment without the transmembrane region of P32 was amplified by PCR, and the E. coli expression vector pPRP-TRUNCP32 was constructed. A fusion protein nearly 31kDa was expressed by IPTG-induced BL21(DE3).

• 以PCR方法扩增了切去跨膜结构的P32基因片段,构建了表达部分P32基因的大肠杆菌表达载体pPRO-TRUNCP32,转化大肠杆菌BL21(DE3),经诱导表达出特异的31kDa融合蛋白,蛋白以包涵体形式存在。