The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation.
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释义
将两种候选蛋白的编码序列分别克隆于上述融合载体,转染Cos?7细胞后的表达产物,可利用抗HA或myc的mAb进行Western?blot和免疫共沉淀。
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