The second part was seguence analysis of the genome of the AEV-NH937.Nine pairs of primers were designed according to published Calnek vaccine strain of AEV. RT-PCR was used to amplify the cDNA of the genome .

• 根据Calnek疫苗株序列设计了9对引物，利用反转录聚合酶链式反应，成功地扩增了AEV-NH937毒株的全基因。